The BD SurePath™ liquid-based Pap test is an FDA approved thin-layer cell preparation process intended for use in the screening and detection of cervical cancer,pre-cancerous lesions, atypical cells and all other cytologic categories as defined by The Bethesda System for Reporting Cervical/Vaginal Cytologic Diagnoses.
FOCUS: Cervical Disease Detection!
BD SurePath™ liquid-based Pap test:
BD SurePath™ Pap test has a unique, easy to use collection process that standardizes the collection process and ensures 100% of the collected sample is sent to the laboratory for processing. No diagnostic cells are discarded in the clinic, as is true with “rinse and swish” systems, which on average can result in the loss of on average, 37% of the collected sample during cell transfer.
The BD FocalPoint™ GS Imaging System combines the automated screening capability of the BD FocalPoint™ Slide Profiler (formerly the AutoPap Primary Screening System) with the precision field location capability of the BD FocalPoint™ GS Review Station. It is intended to assist in cervical cancer screening of BD SurePath™ Pap Test slides to detect evidence of Squamous carcinoma, adenocarcinoma and their precursor conditions.
Here is a video of the slide preperation process
Cell population is homgenized within vial by vortexing.
Density reagent is added to an empty vial.
Most of the specimen is slowly transferred to a vial ontop of the density reagent by an automated processfor further processing (the remaining material is saved for additional testing).
The vial is centrifuged allowing the desinty reagent to filter out debris to the top of the vial while pushing the cells to the bottom.
Most of the debris and density reagent seperated during the centrifuge is decanted.
The vial is centrifuged again, pushing the cells further to the bottom creating a cell pellet and forcing the remaining density reagent to the top.
The remaining density reagent and debris seperated in the second centrifuge is decanted, leaving just the cell pellet.
The cell pellet is vortexed to ensure adequate dispersion of the patients cells.
The Specimen is loaded into an automated processer for further processing.
An automated software controlled machine aspirates the specimen onto a slide, then stains the slide with a papanicolaou stain procedure. The slides are then manually coverslipped.
The Slides are then loaded into the Focal Point device to be triaged by imaging software. Next the device runs its Guidence System (GS) software pinpointing suspicious areas on the slide and once reviewed by a human, the guidence system forces cytotechnicians and pathologists to review these areas by automatically moving their microscopes stage. The software also makes sure a human reviews 100% of the cells on the slide.